Abstract
A flow-injection system with two sample carrier streams, one of them for on-line sucrose inversion, is proposed for the determination of sucrose and total reducing sugar. The method is based on the unselective oxidation of sugars by alkaline hexacyanoferrate(III) at ca. 95°C; the resulting hexacyanoferrate(II) is measured spectrophotometrically after addition of 1,10-phenanthroline and iron(III) sulphate. Two signals are recorded per cycle, reflecting the reducing sugar content of the sample before and after sucrose inversion. A debubber chamber removes the gases produced inside the heated reactors. Boiling water under reflux is used for heating. The effects of experimental variables and possible interferents are reported. The proposed system permits the analysis of about 40 samples per hour (80 measurements). Precise results (r.s.d.<1%) in agreement with those obtained with classical methods are achieved. Simplifications of the system are discussed.
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