Abstract
Four simple, sensitive, accurate and rapid visible spectrophotometric methods (A, B, C and D) have been developed for the estimation of sulfadoxine in pharmaceutical preparations. They are based on the diazotization of sulfadoxine with sodium nitrite and hydrochloric acid followed by coupling withN-(1-naphthyl) ethylenediamine dihydrochloride (Method A) to form pink coloured chromogen, diphenylamine (Method B) to form light pink coloured chromogen, chromotropic acid (in alkaline medium) (Method C) to form orange coloured chromogen, Resorcinol (in alkaline medium) (Method D) to form light orange coloured chromogen and exhibiting absorption maxima (λmax) at 536 nm, 524 nm, 520 nm and 496 nm respectively. The coloured chromogens formed are stable for more than 2 h. Beer’s law was obeyed in the concentration range of 1.0 - 5.0 μg/mL in Method A , 5.0 - 25.0 μg/mL in Method B, 5.0 - 25.0 μg/mL in Method C and 4.0 - 8.0 μg/mL in Method D respectively. The results of the three analysis have been validated statistically and by recovery studies. The results obtained in the proposed methods are in good agreements with labeled amounts, when marketed pharmaceutical preparations are analyzed.
Highlights
Sulfadoxine[1] is chemically 4-amino-N-(5,6-dimethoxypyrimidin-4-yl) benzenesulphonamide (Molecular mass 310.33 g/mol)
Sulfadoxine is an ultra-long-lasting sulfonamide often used in combination with pyrimethamine to treat or prevent malaria
Either drug by itself is only moderately effective in treating malaria, because the parasite Plasmodium falciparum may be able to use exogenous folic acid, i.e. folic acid which is present in the parasite's environment, while in combination, the two substances have a synergistic effect which outbalances that ability[2]
Summary
Sulfadoxine[1] is chemically 4-amino-N-(5,6-dimethoxypyrimidin-4-yl) benzenesulphonamide (Molecular mass 310.33 g/mol). Sulfadoxine is an ultra-long-lasting sulfonamide often used in combination with pyrimethamine to treat or prevent malaria It is used, usually in combination with other drugs, to treat or prevent various infections in livestock. It is no longer recommended as a routine preventative, but only to treat serious malaria infections or to prevent them in areas where other drugs may not work. About 100 mg of sulfadoxine weighed accurately and dissolved in 30 mL of 2 mol Hydrochloric acid in a 100 mL volumetric flask and diluted up to the mark with water (1000 μg/mL). Tablets powder equivalent to 100 mg of the drug weighed accurately and dissolved in 30 mL of 2 mol hydrochloric acid in a 100 mL volumetric flask and allow to sonicate with intermittent shaking for 10 min, cooled and diluted up to the mark with water (1000 μg/mL). The solutions were filtered through Whatman filter paper No 41 and the final concentration of sulfadoxine was brought to 100 μg/mL with water
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