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Abstract
Simple, rapid, cheap and sensitive spectrophotometric method has been described for the determination of zinc in pharmaceutical samples. The method is based on the formation of zinc- 8-Hydroxy quinoline chelate, the maximum absorption (lmax) at 384 nm. The method obeyed Beer's law in the range 1-5 mg/mL and the corresponding molar absorptivity value is 0.01578 ´ 103 L.mol-1.cm-1. The Sandell sensitivity values of limits of detection (LOD) and quantification (LOQ) was 0.381mg/mL and 1.156 mg/mL respectively. The recovery percentage of zinc was found 98.00 %, 98.96 %,99.91%, 97.50%, 98.5% and 99.30 % for (Capsule-13 mg), (Tablet-20 mg), (Tablet-40 mg),(Capsule-50 mg), (Capsule-50 mg) and (insulin vial-0.025 mg) respectively. All variable parameters has been optimized according to ICH guidelines. The limiting concentrations of some cations for interference by Mn(II), Fe(II), Co(II), Ni(II), Cu(II), Cd(II),Sn(II) ,Pb(II), Mg(II), Ca(II) and Ba(II) are reported. The method accuracy was established by comparison with conventional flame atomic absorption spectrometric method by using t-test, ttab.= 2.571, tcal. =0.3231 at 95% confidence level, indicating the absence of systematic errors.
Highlights
Zinc is as ordinary as iron (Williams, Phil, & F.R.S., 1984)
The target of the study is to find out the validity of of the spectrophotometric method for determination of zinc in pharmaceutical samples by using 8-Hydroxyqinoline reagent
The UV-VIS absorption spectra of the zinc- 8HQ chelate was shown in Fig.2, acquired from 350-450 nm with a maximum absorbance 384 nm and an average molar absorptivity (0.01578 103 L. mol.-1cm-1)
Summary
Zinc is as ordinary as iron (Williams, Phil, & F.R.S., 1984) It is a necessary trace element and plays a vital role in normal growth and development (Favier & Hininger-Favier, 2005), many biological functions such as cellular integrity, protein synthesis and nucleic acid metabolism need Zinc. It plays important role in brain development and as an antioxidant (Takeda, 2000) and (Tate, Miceli, & Newsome, 1999) Zinc is a fundamental micronutrient (Maret & Sandstead, 2006). The target of the study is to find out the validity of (system suitability, accuracy, linearity and precision) of the spectrophotometric method for determination of zinc in pharmaceutical samples by using 8-Hydroxyqinoline reagent
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