Spectrophotometric determination of transition elements by cloud point extraction with use laboratory by thiazol azo reagent and applied in environmental samples

Abstract

New azo reagent ( 2-[2- Cefpodoxime proxtel azo]2-paracetamole)(CefAP) prepare that characterization by spectroscopy (UV.-Vis.), (FTIR)' (HNMR) spectrum and (C.H.N). The reagent use to determine the Ni+2 and Co+2 by cloud point extraction(CPE) to determination. That extract as ion pair association complex(CPE) technique. Ni+2 the spectral at Amax =412 nm and Co+2 at λmax = 340 nm. The optimum conditions study like pH = 9, (1%) triton X-100 concentration of TX-100 =0.7 mL optimum time (10 minutes) and heating time =80C° for both ions. Thermodynamic study to Ni+2 and Co+2 respectively parameters ΔH = 0.1908KJ, Δ H = 0.0532 KJ, ΔG = -17.1082KJ, ΔG = -16.1317 KJ and ΔS = 48.4651J, ΔS = 45.69893 J. The data show endothermic reaction. The quantitation limit (LOQ=2.4µg mL-1), (LOQ=5 µg mL-1) and detection limit (LOD=0.24µg mL-1), (LOD=0.5 µg mL-1). Molar absorptivity (ε = 19785 mol-1.cm-1) of metal: reagent (1:1)' (ε = 38977 mol-1.cm-1) of metal: reagent (1:1) by slope analysis to Ni+2 and Co+2 respectively preconcentration and enrichment factors are(14.2and1.899),(14.2and24.0732) with Sandell's sensitivity(3.1 × 10-7µg cm-2) (2.06 × 10-6µg cm-2). A calibration curve linearity range of( 1-20 ppm)and(1-7ppm) a correlation coefficient of 0.9981 and 0.992. The relative standard deviations are 0.79 and 4.64 to Ni+2 and Co+2 respectively The proposed method applied for the determination to Ni+2 and Co+2 in fish, vegetables, and plant leaves. The applications of (CPE) to determination in Ni+2 and Co+2 result that statistical study calculate compound with atomic absorption that no any significant differences at 95%.