Abstract
Uranyl acetate is proposed as a reagent for the spectrophotometric determination of the tetracycline group of antibiotics. The reagent forms orange-red 1:1 complexes with the drugs in N, N-dimethylformamide medium. The complexes show absorption maxima at 414, 406, 419, 405 and 402 nm for tetracycline hydrochloride (TCH), oxytetracycline hydrochloride (OTCH), chlortetracycline hydrochloride (CTCH), doxycycline hydrochloride (DCH) and methacycline hydrochloride (MCH), respectively. Beer's law is valid over the concentration ranges 0–115, 0–120, 0–125, 0–135 and 0–110 μg/ml for TCH, OTCH, CTCH, DCH and MCH, respectively.
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