Abstract
This paper involves a spectrophotometric method for the determination of trace amounts of tetracycline in aqueous solution. The method is based on the coupling of tetracycline with diazotized p-nitroaniline reagent in basic medium to form a violet mono azo dye that is water – soluble, stable and shows maximum absorption at 569 nm. Beer’s law is obeyed over the concentration range 2 – 400 g / 25 ml final volume, i.e., (0.08 – 16) ppm with a molar absorptivity of 7.8 104 l.mol-1.cm-1 and sandell sensitivity index of 6.2 10-3 g cm-2. The method is applied to the determination of the compound in serum and urine by standard addition method in vitro and in vivo (serum and urine) and pharmaceutical preparation (tetracyclline’s ointment).
Highlights
IntroductionTetracycline is the name for the four (tetra) hydrocarbon ring (cycle) derivation (ine) (Wilson et al, 1977)
Tetracycline is the name for the four hydrocarbon ring derivation (Wilson et al, 1977)
The influence of various reaction variables on the colour development is tested to establish the optimum conditions
Summary
Tetracycline is the name for the four (tetra) hydrocarbon ring (cycle) derivation (ine) (Wilson et al, 1977). Tetracycline is an effective antibiotic in treating infections (Talaro and Talaro, 1996), and its absorption is reduced by anti-acids and milk, because it can form insoluble complexes with Ca, Mg, Al and Fe (Alwan and Abou, 1990)
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