Spectrophotometric Determination of Sulfadoxine Drug use Cloud point and Flow injection Methods in Pharmaceutical Formulations

Abstract

New simple sensitive spectrophotometric methods are developed for the estimation of Sulfadoxine (SFD) in pure and pharmaceutical formulations. The first method includes a conversion primary amine to azo-dye by reacting sulfadoxine with sodium nitrite and hydrochloric acid followed by coupling with4-methoxyphenol in alkaline medium to obtain a stable reddish-orange colored dye at λmax495nm. Concentration ranges 0.25-60 μg / mL, obeyed Beer's law, correlation coefficient was 0.9996, molar absorptivity was 0.589×104L.mol-1.cm-1 and the detection limit was 0.157μg/mL. The second method was cloud point extraction (CPE) for estimating trace amount in an aqueous solution that produced from diazotization and measuring with a UV-visible spectrophotometer as are reddish-purple colored product at λmax500 nm. The concentration range obeyed the Beer's law was 0.25-6μg / mL, correlation coefficient was 0.9998, molar absorptivity was 0.877×105L.mol-1.cm-1, detection limit was 0.023μg/mL, pre-concentration factor was 25 and Distribution coefficient(D) was 320.88. The last method was flow injection analysis it’s simple for estimation the sulfadoxine. The concentration range was1-150μg / mL, obeyed Beer's law,the correlation coefficient was 0.9997, molar absorptivity was 0.273×104L.mol-1.cm-1 and the detection limit was 0.375μg/mL. The offered methods were successful, useful for estimating sulfadoxine in traditional medications