Abstract
The spectrophotometric method of Haugaard and Damm (1) for estimating the protein content of skimmilk at wavelength 277 mμ was modified for raw and pasteurized milk, sterile concentrate, and cream as follows: Add 0.5ml of butylamine to 0.5ml of milk, heat to 65C for 5min, add 25ml of a mixture of 0.3% each of disodium ethylenediamine—tetraacetate (EDTA) and sodium lauryl sulfate (SLS), add five drops of hydrochloric acid and filter. Add one drop of 30% sodium hydroxide solution and read the optical density at 280 mμ.For milk changed by the Maillard-type reaction, add 0.5ml of butylamine to 0.5ml of milk, heat to 65C and add two drops of sodium borohydride. The remainder of the procedure is the same.Results for whole milk, sterile concentrated milk, and cream agreed with the Kjeldahl method.
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