Abstract
In this paper, a novel spectrophotometric method has been established to determine perphenazine using the detection system of potassium ferricyanide–Fe(III). In the presence of potassium ferricyanide, it has been demonstrated that Fe(III) is reduced to Fe(II) by perphenazine at pH 4.0. In addition, soluble prussian blue (KFe III[Fe II(CN) 6]) is produced by the reaction between the in situ formed Fe(II) and potassium ferricyanide. The absorbance of soluble prussian blue is measured at the absorption maximum of 735 nm, and the amount of perphenazine can be calculated based on this absorbance. The absorbance is linear to perphenazine concentration in the ranges of 0.05–25.00 μg/mL with correlation coefficients of 0.9997, and the linear regression equation is A = 0.0069 + 0.1285 C (μg/mL). The detection limit (3 σ/ k) is 0.049 μg/mL, and the relative standard deviation (R.S.D.) is 0.86% ( n = 11). Moreover, the apparent molar absorption coefficient of indirect determination of perphenazine is 5.2 × 10 4 L/mol cm. The parameters with regard to determination are optimized, and the reaction mechanism is discussed. This method has been successfully applied to the determination of perphenazine in pharmaceutical and serum samples. Analytical results obtained with this novel assay are satisfactory.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
