Abstract
An accurate, simple, and sensitive indirect spectrophotometric method which proposed and developed for the determination of paracetamol in different pharmaceutical preparations. The proposed method was based on acid hydrolysis of PAR to produced p-aminophenol (PAP), PAP was diazotization with nitrite ion to form the corresponding diazonium salt, followed by coupling with histidine reagent in alkaline medium to produced azo dye that showed maximum absorbance at 430 nm. Beer's law was obeyed in the concentration rage of 10-500 µg/20 ml (i.e. 0.5-25 ppm). The molar absorpitivity and Sandell's sensitivity of the dye were. 1.118×104 l.mol-1.cm-1and 0.0135 µg.cm-2 respectively. The method successfully has been applied for the determination of PAR in pure form,and its pharmaceutical preparations (tablets, syrup and injection). ).
Highlights
Paracetamol [N-(4-hydroxy-phenyl) acetamide] is a chemical compound and drug that is commonly used for headaches, other minor aches and pains of the management more sever pains where it gives room for additional non-steroidal anti-inflammatory drugs which have to be used at lower doses, for minimizing side effect (Chandka et al, 2013)
The present work is devoted to indirect spectrophotometric method for the determination of paracetamol included the diazotisation of p-amiophenol which results from acid hydrolysis of PAR, followed by coupling reaction with histidine reagent in the presence of alkaline medium to yield an azo dye that shows maximum absorbance at 340 nm, that has been applied successfully for the determination of paracetamol in pharmaceutical preparations
The maximum absorbance value of the colored azo dye formed in an alkaline medium of 100 μg.ml-1 paracetamol (PAR) solution with histidine reagent was observed at 430 nm, in the final volume was brought to 20 ml with distilled water
Summary
Paracetamol [N-(4-hydroxy-phenyl) acetamide] is a chemical compound and drug that is commonly used for headaches, other minor aches and pains of the management more sever pains where it gives room for additional non-steroidal anti-inflammatory drugs which have to be used at lower doses, for minimizing side effect (Chandka et al, 2013). The present work is devoted to indirect spectrophotometric method for the determination of paracetamol included the diazotisation of p-amiophenol which results from acid hydrolysis of PAR, followed by coupling reaction with histidine reagent in the presence of alkaline medium to yield an azo dye that shows maximum absorbance at 340 nm, that has been applied successfully for the determination of paracetamol in pharmaceutical preparations.
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