Abstract
A simple, rapid, accurate and precise spectrophotometric method is proposed for the determination of nitrazepam in pure form. The method is based on the reduction of nitrazepam by zinc powder in concentrated hydrochloric acid medium followed by diazotization of reduced nitrazepam and coupling with N-(1-naphthyl)ethylenediamine dihydrochloride (NEDA) to give a pink coloured product which is stable, water-soluble and has a maximum absorption at 564 nm with a molar absorptivity of 1.633×10 3 l.mol -1 .cm -1 . Beer's law is obeyed in the concentration range of 10 to 500 µg of nitrazepam in a final volume of 25 ml. ــــــــــــــــ ــــــــــــــــ ــــــــــــــــ ــــ
Highlights
Six benzodiazepines play prominent roles in the therapy of epilepsy
During the investigation, 100 μg of reducing nitrazepam is taken and the final volumes are brought to 25 ml with distilled water
Effect of sodium nitrite amount with the time Different amounts of the 0.5% NaNO2 solution are added and the time needed to complete the diazotization of reducing form of nitrazepam is studied by standing of the solutions after adding sodium nitrite solution for different times, with occasional shaking, the other reagents are added and the absorbance is measured against the reagent blank .The results indicate that complete diazotization of nitrazepam occurs after 3 min. when 1 ml of 0.5% NaNO2 solution is added because it gives the higher sensitivity, it has been selected for subsequent experiments
Summary
Six benzodiazepines play prominent roles in the therapy of epilepsy. benzodiazepines are chemically quite similar with a suitable structural, there are differences in their activities. Procedure and calibration graph To a series of 25 ml calibrated flasks, an increasing volume covering the concentration range (1-500) μg .25ml-1 of reducing form of nitrazepam solution are transferred, followed by the addition of 1 ml of 1N of hydrochloric acid and 1 ml of 0.5% sodium nitrite solution and, shaking occasionally for 3 min, a 3 ml of 1% sulphamic acid is added, with occasional shaking and standing for 3 min.
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