Spectrophotometric Determination of Mesalazine by Oxidation and Shortening Using Methyl Orange Dye and Application to Pharmaceutical Preparations

Abstract

A rapid, sensitive and indirect spectral method was developed to estimate the mesalazine in the aqueous solution, as the method relied on the oxidation and reduction reaction of the mesalazine oxidation with an increase (calculated) from the oxidizing agent of bromate - bromide mixture in the medium of hydrochloric acid. The remaining (non-reducing) concentration then reacts. With a constant amount of methyl orange dye leads to color bleaching. The residual dye was followed at 507 nm wavelength. The relationship was linear between residual pigment absorption and increased drug concentration, and the micrographic quantities of mesalazine that followed Beer ٬ s law limits were estimated from 0.5 to 12.5 µg /ml with molar absorptivity 2.03 x 104 l.Mol-1.. cm-1 and Sand ell’s sensitivity index (0.0075357) µg / cm2 . A relative standard deviation whose value does not exceed 0.690% and the C recovery value not less than 99.05%. The method was successfully applied to the pharmaceutical preparations as tablets and it was compatible with the original content of the pharmaceuticals and with the results of the standard addition method.