Abstract
A spectrophotometric continuous flow-injection, merging zones manifold including an enzyme reactor was used for the determination of magnesium ion in serum by exploiting the activating effect of Mg(II) on the hydrolysis of o-nitrophenyl-β- d-galactopyranoside by β- d-galactosidase immobilized on controlled-pore glass. The advantageous features of the proposed method (determination range 5–20 μmol 1 −1, within-run and between-run relative standard deviations of 0.78–2.91% and 1.7–3.21%, respectively) allow the determination of the analyte in serum samples with good recoveries (96–113%). The results obtained were consistent with those provided by the conventional atomic absorption spectrometric method.
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