Abstract
A spectrophotometric method for the assay of lamivudine in pure form and in dosage form was developed in this study. The method was based on charge-transfer complex formation between the drug, which acted as n-donor while chloranilic acid and 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ) acted as a p-acceptor in a non-aqueous solvent in each case. Chloranilic acid was found to form a charge-transfer complex in a 1:1 stoichiometry with lamuvudine (lamivudine-chloranilic acid) with a maximium absorption band at 521 nm. Also, DDQ was found to form a charge-transfer complex in a 1:1 stoichiometry with lamivudine (lamivudine-DDQ) with a maximium absorption band at 530 nm. The pH was obeyed at acid range. The complexes obeyed Beer’s law at a concentration range of 0.04 - 0.28 mg/ml. The thermodynamic parameters calculated at different temperatures included the molar absorptivity, association constant, free energy change, enthalpy and entropy. The proposed method has been conveniently applied in the analysis of commercially available lamivudine tablet with good accuracy and precision.
Highlights
It is an antiretroviral drug belonging to the class called nucleoside reverse transcriptase inhibitors (NRTIs)[1]
Lamivudine-Chloranilic Acid Complex One tablet formulation of lamivudine equivalent to 150 mg of lamivudine was crushed in a crucible and dissolved in a 50 mL standard flask with methanol to extract the active pharmaceutical ingredient (API)
The interaction between lamivudine and chloranilic acid is as shown in Scheme 1
Summary
It is an antiretroviral drug belonging to the class called nucleoside reverse transcriptase inhibitors (NRTIs)[1]. It exhibits potent antiretroviral activity [2]. It was indicated that combination therapy of lamivudine with zidovudine is associated with substantial persistent increase in CD4 cell counts and decreases in HIV RNA as measured by polymerase chain reactions [3]. Chloranilic acid, DDQ and other -acceptors have been variously utilized in the spectrophotometric assay and analysis of many drugs by charge-transfer complexation [4,5,6,7,8,9]. The method has not been adopted in the analysis of this drug both in pure sample and in pharmaceutical formulations
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