Abstract
Spectrophotometric Determination of L-Ascorbic Acid in Pharmaceuticals Based on Its Oxidation by Potassium Peroxymonosulfate and Hydrogen Peroxide
Highlights
L-Ascorbic acid (2-oxo-L-threo-hexono-1,4-lactone-2,3enediol) is an essential vitamin which participates in many different biological processes
The aim of this work was to develop two simple, accurate, and sensitive spectrophotometric methods for the determination of ascorbic acid in pharmaceuticals with background correction based on the oxidation of L-ascorbic acid by peroxymonosulfate or hydrogen peroxide in the presence of Cu(II) catalyst
B Because L-ascorbic acid was unstable at pH > 5.0, the effect of pH on the oxidation of L-ascorbic acid (16.00 μg mL–1) by hydrogen peroxide was investigated over the range 3.00–4.50 in the presence of 0.0456 M H2O2
Summary
L-Ascorbic acid (2-oxo-L-threo-hexono-1,4-lactone-2,3enediol) is an essential vitamin which participates in many different biological processes. It occurs naturally in most fruit juices and vegetables. Many analytical methods have been reported in the literature for the determination of the ascorbic acid contents in different pharmaceutical products, foods and biological fluids. These include spectrophotometric,[1,2,3,4] highperformance liquid chromatographic,[5] electrochemical,[6] fluorimetric[7] and chemiluminescent[8] methods. The enzymatic methods are simple and highly specific for L-ascorbic acid, a major obstacle to the wide usage of these methods is the high costs of purified enzymes
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