Abstract
Abstract To improve the procedure of photometric determination of iron in human serum, Torii’s method recently proposed for the determination of iron was applied to serum. After the removal of protein from serum, the iron in serum is reduced, the pH of the solution is adjusted to 4.5, the color-developing reagent is added, and the excess of the reagent is removed by extraction with carbon tetrachloride. The green solution thus obtained is effective for spectrophotometric determination. The interference of copper is avoided by the addition of thiosulfate, and the substances other than copper remaining after the removal of protein from serum do not give any appreciable interference.
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