Spectrophotometric determination of Cu(II) in soil and vegetable samples collected from Abraha Atsbeha, Tigray, Ethiopia using heterocyclic thiosemicarbazone.

Daniel Admasu,Kebede Nigussie Mekonnen,Desam Nagarjuna Reddy

doi:10.1186/s40064-016-2848-3

Daniel Admasu, Kebede Nigussie Mekonnen + Show 1 more

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https://doi.org/10.1186/s40064-016-2848-3

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Journal: SpringerPlus	Publication Date: Jul 26, 2016
Citations: 19	License type: CC BY 4.0

Affiliation: Mekelle University

Abstract

Two selective and sensitive reagents, 2-acetylpyridine thiosemicarbazone (2-APT) and 3-acetylpyridine thiosemicarbazone (3-APT) were used for the spectrophotometric determination of Cu(II). Both reagents gave yellowish Cu(II) complex at a pH range of 8.0–10.0. Beer’s law was obeyed for Cu(II)–2-APT and Cu(II)–3-APT in the concentration range of 0.16–1.3 and 0.44–1.05 µg/mL, respectively. The molar absorptivity and of Cu(II)–2-APT and Cu(II)–3-APT were 2.14 × 104 at 370 nm, and 6.7 × 103 L/mol cm at 350 nm, respectively, while the Sandell’s sensitivity were 0.009 and 0.029 µg/cm2 in that order. The correlation coefficient of the standard curves of Cu(II)–2-APT and Cu(II)–3-APT were 0.999 and 0.998, respectively. The detection limit of the Cu(II)–2-APT and Cu(II)–3-APT methods were 0.053 and 0.147 µg/mL, respectively. The results demonstrated that the procedure is precise (relative standard deviation <2 %, n = 10). The method was tested for Cu(II) determination in soil and vegetable samples. Comparisons of the results with those obtained using a flame atomic absorption spectrophotometer for Cu(II) determination also tested the validity of the method using paired sample t test at the 0.05 level showing a good agreement between them.

Highlights

Copper can be considered either essential or hazardous to life and plays a substantial role in the environment (Fu and Yuan 2007; Horstkotte et al 2012; Gouda and Amin 2014; Tarighat 2016)
Several analytical techniques have been used for determination of copper, including atomic absorption spectrometry, atomic emission spectrometry, electroanalytical techniques, spectrophotometry, inductive coupled plasma-emission spectrometry, inductive coupled plasma-mass spectrometry, flow injection diode array spectrophotometry and X-ray fluorescence spectrometry (Pinto et al 2004; Kruanetr et al 2008; Gouda and Amin 2014; Nalawade et al 2015; Tarighat 2016)
The IR spectrum of 2-Acetylpyridine thiosemicarbazone (2-APT) exhibits absorption bands corresponding to υ (N–H, asym), υ (N–H, sym), υ (C–H, pyridine), υ (C=N, Schiff ’s base), υ (C–C, pyridine), δ (C–H, aromatic ring), υ (N–H, primary amide), υ (C=S) and δ (C–H, aromatic ring) at 3373 (m), 3261 (m, br), 3183 (s), 1608 (s), 1501 (s), 1466 (s), 1244 (w), 1086 (m) and 783 (m) cm−1, respectively

Summary

Introduction

Copper can be considered either essential or hazardous to life and plays a substantial role in the environment (Fu and Yuan 2007; Horstkotte et al 2012; Gouda and Amin 2014; Tarighat 2016). Some representative examples are S,S′-bis(2-aminophenyl)oxalate (Nohut et al 1999), naphthazarin (Chaisuksant et al 2000), 3-{2-[2-(2-hydroxyimino-1-methyl-propylideneamino)-ethylamino]-ethylimino}-butan-2-one oxime (H2mdo) (Dalman et al 2002), p-anisidine with N,N-dimethylaniline (DMA) (Ohno et al 2003), 1-phenyl-1,2-propanedione-2-oxime thiosemicarbazone (PPDOT) (Reddy et al 2003), di-2-pyridyl ketone benzoylhydrazone (dPKBH) (Pinto et al 2004), 3,3,5,5-tetramethybenzidine (TMB) (Di et al 2005), thiomichlersketone (TMK) (Fu and Yuan 2007), 1-(2-thiazolylazo)-2-naphthol (Niazi and Yazdanipour 2008), benzyloxybenzaldehyde-4-phenyl-3-thiosemicarbazone (Prathima et al 2010), formazan dye Zincon (Sabel et al 2010), 1-(2,4-dinitro aminophenyl)-4,4,6trimethyl-1,4-dihydropyrimidine-2-thiol (Kamble et al 2011), sodium diethyldithiocarbamate (Na-DDTC) (Uddin et al 2013) and 2-amino-4-(m-tolylazo) pyridine3-ol (ATAP) (Gouda and Amin 2014)

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