Abstract
A sensitive spectrophotometric method for the determination of cholesterol in serum samples was described. 4-Hydroxybenzaldehyde was used for spectrophotometric determination of cholesterol. The method based on the formation of coloured compound showed maximum absorbance at 525 nm. Beer ’ s law was obeyed in the concentration range 1.0 – 30.0 µg/ml with detection limit 0.14 µg/ml. The method was applied for the analyses of cholesterol in human serum samples. The results were compared with biolabo kit and biolis automatic instrument as a standard method which shows good agreement between them.
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