Abstract
Sensitive, economic, simple and accurate spectrophotometric method for estimation of ampicillin in bulkand dosage form is described. Ampicillin was oxidized by sodium hypochlorite and coupling with sulfanilicacid in the presence of sodium hydroxide to obtain a stable yellow colored chromogen which exhibit amaximum absorption (?max) at 400 nm. The optimum conditions were carefully evaluated .Plot of absorbance against concentration was linear over the range (50-300 µg.ml-1). This method was applied tothe estimation of ampicillin in pure drug and commercial formulations successfully.
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