Spectrophotometric determination of ajmaline and brucine by Folin Ciocalteu’s reagent

Abstract

Arapid and simple spectrophotometric procedure is described for the determination of ajmaline and brucine. The method is based on the development of blue colored product due to reduction of tungstate and/or molybdate in Folin Ciocalteu?s reagent (FCR) by ajmaline and brucine in alkaline medium. The color is stable for more than 48 h. The chromogenic reaction has ?max at 540 nm with molar absorptivity 1.64 x 104 and 2.37 x 103 l mol-1 cm-1 in the Beer?s law range 1?8 ?g ml-1 and 10?100 ?g ml-1 for ajmaline and brucine, respectively.