Abstract
Two selective and sensitive spectrophotometric methods have been developed for the estimation of adefovir dipivoxil in bulk and pharmaceutical preparations. Adefovir dipivoxil was subjected to acid hydrolysis and the hydrolysed product used for the estimation. The methods are based on the reaction with 3-methyl-2-benzothiazolinone hydrazone in the presence of ferric chloride, to form a colored species with absorption maxima at 627 nm. The second method is based on the reaction of drug with 1,2 naphthaquinone -4-sulphonic acid sodium salt, under alkaline conditions which absorbs maximally at 454 nm. Beers law is obeyed in the concentration range of 5-25 µg/mL and 2-10 µg/mL respectively. These methods were extended to pharmaceutical formulations and there was no interference from excepients and diluents. The analytical parameters were evaluated.
Highlights
Adefovir dipivoxil is an antiviral drug and chemically [ 2-(6-aminopurine–9yl) ethoxy methyl(2,2-dimethyl propanoyloxy methoxy phosphoryl) oxy methyl 2,2-dimethyl propanoate[1,2]
The colored species was stable for 2.0 h and the amount of the unknown sample was computed from its calibration graph
The tablet powder equivalent to 100 mg of adefovir dipivoxil was transferred into 100 mL volumetric flask containing 50 mL of methanol and the flask was kept for ultrasonication for 5 min
Summary
Adefovir dipivoxil is an antiviral drug and chemically [ 2-(6-aminopurine–9yl) ethoxy methyl(2,2-dimethyl propanoyloxy methoxy phosphoryl) oxy methyl 2,2-dimethyl propanoate[1,2]. Adefovir is an acyclic nucleotide analong of adenosine monophosophate[3] It is a new generation antiviral drug which is active in-vitro against HBV. It is indicated for the treatment of chronic hepatitis-B and HIV. Ferric chloride 0.2% was freshly prepared by dissolving 200 mg of ferric chloride in 100 mL of distilled water. Folins reagent was prepared by dissolving 1 g of sodium 1, 2-naphthaquinone-4- sulphonate in 100 mL of distilled water. Sodium hydroxide solution was prepared by dissolving 5 g in 100 mL of distilled water
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