Abstract
A spectrophotometric- reverse flow injection analysis (rFIA) method has been proposed for the determination of Nitrazepam (NIT) in pure and pharmaceutical preparations. The method is based upon the coupling reaction of NIT with a new reagent O-Coumaric acid (OCA) in the presence of sodium periodate in an aqueous solution. The blue color product was measured at 632 nm. The variation (chemical and physical parameters) related with reverse flow system were estimated. The linearity was over the range 15 - 450 µg/mL of NIT with detection limits and limit of quantification of 3.425 and 11.417 µg mL-1 NIT,respectively. The sample throughput of 28 samples per hour was achieved. Suggested method was successfully applied for the determination of NIT in its dosage forms.
Highlights
Nitrazepam (NIT) (7-nitro-5-phenyl-1H-benzo[e][1,4]diazin-2(3H)-one, M. wt. =281.3 g/mol), is a hypotic agent that belongs to the benzodiapine class
One of the flow injection analysis modes is reverse flow injection analysis, in which the reagent is injected into the sample stream which is reversed to the usual FIA when the sample is injected
The second channel was used to flow sodium periodate solution, while the reagent OCoumaric acid (OCA) was injected through the injected valve it was mixed with the stream of drug and oxidant and the mixing completed in the reaction coil
Summary
Benzo[e][1,4]diazin-2(3H)-one, M. wt. =281.3 g/mol), is a hypotic agent that belongs to the benzodiapine class. NIT is used for healing the sedative effects of insomnia [1], as well as anxiolytic, amnestic, anticovulsant and skeletal muscle relaxant properties. It has been used in the treatment of stress associated with disorders [2,3]. The present investigation method offers an improvement of the simple spectrophotometric-rFI technique for the estimation of NIT in both pure and pharmaceutical forms as tablet depending on the oxidative coupling reaction between NIT and OCoumaric acid as a new chromogenic agent in the presence of sodium periodate in an aqueous medium to produce a blue-colored product
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