Abstract
A rapid, simple, and sensitive spectrophotometric and high performance liquid chromatographic methods have been developed for the quantitative determination of metoclopramide hydrochloride MCP in both pure and dosage forms. The spectrophotometric method is based on diazotization of MCP and then coupling with 2,4-dihydroxyacetophenone in alkaline medium. The resulting azodye exhibits maximum absorption at 450 nm with a molar absorptivity of 2.48 X 104 l.mol-1.cm-1. Beer's law is obeyed over the range 10-300µg/25 ml, i.e., 0.4-12 ppm with a relative standard deviation (RSD)of better than±1.092% and relative error better than -1.1%. HPLC method has been developed for the measurement of MCP, the analysis was achieved on a C8 column using acetonitrile, water, and methanol in the ratio of 40:50:10 (V:V:V) (in the presence of 1% of sodium acetate) as a mobile phase by isocratic elution at 1 ml/min. flow rate, and detection was done spectrophotometrically at 308 nm. A linear relationship is obeyed over the range 0.18-0.8 ppm with a relative standard deviation (RSD) of better than 3.7% and relative error better than -1.6%. Both methods were applied successfully to the assay of MCP in pharmaceutical preparations in the form of syrup, injection, and tablet.
Highlights
Metoclopramide (MCP) is a white or almost white, odorless, crystalline powder (m.p. about 185°C) very soluble in water, alcohol freely, partially insoluble in ether. (British Pharmacopoeia, 2008)
Various spectrophotometric methods for the determination of metoclopramide have been reported among these, the spectrophotometric determination of (MCP) by coupling of (MCP) with the oxidized promethazine in acetic acid medium to form a blue product with maximum absorption at 596 nm (Ahmad and Ali, 2006)
Visible spectrophotometric method for the estimation of metoclopramide has been developed by the reaction of (MCP) with 4-dimethylaminobenzaldehyde (Ehrlich reagent) to produce a yellow dye with maximum absorption at 438 nm (Patel et al, 2006)
Summary
Metoclopramide (MCP) is a white or almost white, odorless, crystalline powder (m.p. about 185°C) very soluble in water, alcohol freely, partially insoluble in ether. (British Pharmacopoeia, 2008). Metoclopramide (MCP) is a white or almost white, odorless, crystalline powder (m.p. about 185°C) very soluble in water, alcohol freely, partially insoluble in ether. (MCP) is: 4-amino-5-chloro-N-(2-diethylaminoethyl)-2-methoxybenzamide monohydrochloride monohydrate (Sweetman et al, 2005). Various spectrophotometric methods for the determination of metoclopramide have been reported among these, the spectrophotometric determination of (MCP) by coupling of (MCP) with the oxidized promethazine in acetic acid medium to form a blue product with maximum absorption at 596 nm (Ahmad and Ali, 2006). Visible spectrophotometric method for the estimation of metoclopramide has been developed by the reaction of (MCP) with 4-dimethylaminobenzaldehyde (Ehrlich reagent) to produce a yellow dye with maximum absorption at 438 nm (Patel et al, 2006)
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