Abstract
Samples containing between 1 and 50 nmol of hydroperoxides in oxygen-free methanol-acetic acid containing I − were incubated at 50°C. This ensured reduction of all hydroperoxides tested in 15 min. Addition of sufficient Cd acetate to combine with the remaining I − allowed reading of absorbance of the I 3 − produced in open cuvettes. The absorbance was stable for several hours. It had a maximum at 358 nm with molar extinction coefficient of 2.97 × 10 4 1 mol −1 cm −1. Sensitivity of the assay could be improved by injecting the I 3 − into a C-18 HPLC column eluted with methanol-water-acetic acid solution. Both methods are potentially suitable for assay of hydroperoxides in a wide range of biological materials.
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