Abstract
SummaryA spectrophotometric method for assaying minute quantities of sugar in solution has been modified for use on nectaries of plants and on insects which gather nectar. A flower is rinsed (or an insect crushed) in a measured amount of water; 1 ml of sample solution is added to 1 ml of 5% phenol and 5 ml concentrated sulphuric acid. After 45–60 min the absorbance at 490 nm is measured with a spectrophotometer, and translated into weight of sugar by means of a standard curve. Hundreds of samples can be gathered each day, frozen, and analysed (100/day) during a slack season. As an example, results are given for the sugar content of Tilia nectar as the flower ages.
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