Abstract
Hydroxysteroid dehydrogenase (HSD) activity was determined spectrophotometrically on homogenates of the pronephros, the opisthonephros, and the gonads of metamorphosing, immature, and mature adult male and female brook lampreys ( Lampetra planeri Bloch), employing pregnenolone, dehydroepiandrosterone, androsterone, epiandrosterone, and 3β,17β-dihydroxy-5α-androstane as substrates and NAD or NADP as coenzymes in October, December, February, and April. With NADP a higher HSD activity was observed in the majority of the assays. In larval brook lampreys a NAD-dependent HSD activity was detected in presumed adrenocortical tissue (PAT) homogenates with pregnenolone and 3β,17β-dihydroxy-5α-androstane and in gonad homogenates only with 3β,17β-dihydroxy-5α-androstane. With 3β,17β-dihydroxy-5α-androstane as substrate the highest enzyme activities were measured in PAT and gonad homogenates from animals of all investigated life stages. A varying intensity of HSD activity in the examined tissue homogenates probably dependent on stages of the individual development was observed.
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