Abstract
A new thiol weak-fluorescence probe, 5-maleimidyl-2-( m-methylphenyl)benzoxazole (MMPB), gives a highly fluorescence product in the presence of Cys. In this paper, MMPB has been developed for the fluorimetric determination of cysteine (Cys). At λ ex/ λ em=305.6/425.6 nm, the linear range is from 0 to 3.3×10 −7 mol l −1 and the detection limit ( σ=3) of 6.2×10 −10 mol l −1. The main advantage of this method lies in the relative high selectivity compared with the methods using other N-substituted maleimide type of thiol reagents, in which 0.15-fold (molar ratio) of GSH is allowed and most of other amino acids at 100-fold (molar ratio) level had no obvious effect on the results. The proposed method has been applied to the determination of Cys in real samples.
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