Spectroelectrochemical Determination of Isoprenaline in a Pharmaceutical Sample.

Julia Carazo,Fabiola Olmo,Alvaro Colina,Aranzazu Heras,Jesus Garoz-Ruiz

doi:10.3390/s20185179

Abstract

UV/Vis absorption spectroelectrochemistry (SEC) is a multi-response technique that has been commonly used for the characterization of materials and the study of reaction mechanisms. However, it has been scarcely used for quantitative purposes. SEC allows us to obtain two analytical signals simultaneously, yielding a dual sensor in just one experiment. In the last years, our group has developed new devices useful for analysis. In this work, a SEC device in parallel configuration, based on optical fibers fixed on screen-printed electrodes, was used to determine isoprenaline in a commercial drug, using both, the electrochemical and the spectroscopic signals. In this commercial drug, isoprenaline is accompanied in solution by other compounds. Among them is sodium metabisulfite, an antioxidant that strongly interferes in the isoprenaline determination. A simple pretreatment of the drug sample by bubbling wet-air allows us to avoid the interference of metabisulfite. Here, we demonstrate again the capabilities of UV/Vis absorption SEC as double sensor for analysis and we propose a simple pretreatment to remove interfering compounds.

Highlights

Adrenergic receptors are targets of different catecholamines stimulating the sympathetic nervous system
Isoprenaline or isoproterenol (IP) is a non-selective sympathomimetic β-adrenergic agonist drug that acts on the β-adrenoreceptors. β-adrenergic agonists imitate the behavior of neurotransmitters that belong to the catecholamine family such as adrenaline or noradrenaline
We propose for the first time to the best of our knowledge, the determination of IP with spectroelectrochemistry (SEC)

Summary

Introduction

Adrenergic receptors are targets of different catecholamines stimulating the sympathetic nervous system. The low sensitivity and the large amount of time required to carry out these analyses have led to the development of several electrochemical methods [1,2,3,7,15,16]. Many electrochemical methods proposed to quantify IP require the modification of the electrode surface to achieve the sensitivity and/or selectivity needed. Different materials, such as polymers [15] or nanostructures like multiwalled carbon nanotubes [3,5,17,18], graphene [7], hollow carbon spheres [16], or iridium oxide nanoparticles [19], have been proposed to modify the electrode

Methods

Results

Conclusion