Abstract
Full-field optical coherence microscopy (FFOCM) utilizes coherence gating to obtain high-resolution optical sections in thick tissues. FFOCM is an attractive technology for endoscopic microscopy at the cellular level since it does not require a high NA objective lens or beam scanning and is therefore particularly amenable to miniaturization. In this manuscript, we present a novel scheme for conducting FFOCM that utilizes spectrally modulated, spatially incoherent illumination and a static Linnik interferometer. This approach is advantageous for endoscopic microscopy since it allows FFOCM to be conducted through a single multimode fiber optic imaging bundle and does not require moving parts in the endoscope probe. Images acquired from biological samples in free space demonstrate that this new method provides the same detailed microscopic structure as that of conventional FFOCM. High-resolution images were also obtained through a multimode fiber bundle, further supporting the potential of this method for endoscopic microscopy.
Highlights
Endoscopic imaging at the subcellular level has a wide variety of potential clinical applications, including the early detection of dysplasia/cancer within internal organ systems
Full-field optical coherence microscopy (FFOCM) is capable of obtaining isotropic, cellular-level resolution images in tissue without beam scanning
It is an excellent candidate for endoscopic microscopy of internal organ systems of living human patients
Summary
Endoscopic imaging at the subcellular level has a wide variety of potential clinical applications, including the early detection of dysplasia/cancer within internal organ systems. Bocarra et al [5], demonstrated that implementing OCT with a light source having both low temporal coherence and low spatial coherence can provide high resolution in three-dimensions with relatively low-NA lenses and without requiring rapid beam scanning[6,7]. This approach, known as full-field optical coherence microscopy (FFOCM), has generated striking images of subcellular structure in biological tissues [8,9,10].
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