Abstract
The interactions of Cu2+ with urease were investigated by fluorescence, UV/vis, CD, synchronous fluorescence, and three-dimensional fluorescence spectra techniques. Cu2+ effectively quenched the intrinsic fluorescence of urease via static quenching. The binding constant KA, the binding site n and the thermodynamic parameters are obtained. The process of binding Cu2+ to urease was a spontaneous molecular interaction procedure with electrostatic interaction. The conformation of urease was discussed by UV/vis, CD, synchronous and three-dimensional fluorescence techniques.
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Schedule a callMore From: Spectrochimica Acta Part A: Molecular Spectroscopy
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