Abstract

The pathway for the phototransformation of the red-light-absorbing (Pr) to the far-red-light-absorbing (Pfr) form of native phytochrome A from pea was investigated by low temperature spectrophotometry. Four intermediates between Pr and Pfr were recognized. The first intermediate (lumi-R) was stable below −100°C. The other intermediates and Pfr appeared during warming of lumi-R to −80 °C (meta-Ra), −40 °C (meta-Rb′), −20 °C (meta-Rc) and 0 °C (Pfr). Each intermediate (lumi-R, meta-Ra and meta-Rc) and Pr was photointerconvertible as a two-component system at the temperature at which the intermediate had initially appeared. Meta-Rb′ is also photoconvertible to Pr. Prolonged red light irradiation of meta-Rb′ at −40 °C resulted in appearance of another intermediate (meta-Rb).

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