Abstract
The DNA binding behavior of potentially bisintercalating ligand 1,4-bis(( N-methyl quinolinium-4-yl)vinyl)benzene was studied by spectrophotometric titration, circular dichroism and matrix-assisted laser desorption-ionization-time-of-flight (MALDI-TOF) mass spectrometry. The formation of large DNA–ligand aggregates observed at low DNA concentration was ascribed to the interstrand cross-linking due to the bisintercalation and/or electrostatic interactions. On the other hand, a monointercalation was observed at higher DNA concentration and in the presence of higher content of NaCl. Intercalative DNA–ligand complex was featured by red shifted absorption band, modest hypochromicity and the presence of induced CD signal. MALDI-TOF mass spectra of short oligonucleotide–ligand systems revealed the formation of 1:1 complexes of the ligand with duplex and single-stranded oligonucleotides as well as a higher molecular weight species.
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