Spectral investigations to the effect of bulk and nano ZnO on peanut plant leaves

Abstract

The potential variation in peanut plant leaves were examined by the application of bulk and nano ZnO through presowing method. ZnO nanoparticles synthesized by chemical route and were characterized using x-ray diffraction, atomic force, scanning electron and transmission electron microscopy. ZnO nanoparticles and bulk counterpart are applied to the peanuts by presowing method in two concentrations of 500 ppm and 4000 ppm. Fourier transform infrared spectra exhibit variation in most prominent peaks ∼2923, ∼1636, ∼1033 cm−1 due to ZnO stress in both nano and bulk form, which was supported from the calculated mean ratio of the peak intensities for various frequency region and total band area calculation for various band region. The infrared spectra were further processed by de-convolution and curve fitting analysis to examine the variation in secondary structure protein of leaf samples stressed with ZnO bulk and nanoparticles. Multivariate analyses shows the successive factors account for decreasing amounts of residual variance using two factors for the wave numbers 1633, 1648, 1656, 1666, 1673 and 1684 cm−1 assigned to protein secondary structure of leaf samples.