Spectral Interrogation based SPR Sensor for Blood Glucose Detection with Improved Sensitivity and Stability

Abstract

A sensor chip for specific detection of blood glucose was developed. The sensor utilized a surface-plasmonresonance (SPR) setup on a prism based Kretschmann configuration utilizing spectral interrogation scheme. Selfassembled monolayers (SAMs) based preparation provided the chip stability due to covalent bonds and hence it can be reused for multiple times. Such a scheme can be advantageous in continuous monitoring of blood glucose, without changing the chip. Control experiments were performed without molecular recognition layer to confirm the performance of the sensor. Furthermore, the measurements were performed on blood serums and compared with that of a conventional glucometer. The present sensor has the advantages of improved sensitivity (0.14 nm/(mg/dl)) and stable response for 3 months, which are better compared to existing reports.