Abstract
Super-resolution imaging at single-molecule level distinguishes each molecule within a diffraction-limited volume and precisely localizes its centroids. The foregoing can be achieved by monitoring the spectral blue shift of each quantum dot (QD) with a less than 50nm-long DNA ruler under a spectral imaging microscope. The presence of two QDs can be revealed based on the fact that two similar-sized (color) QDs asynchronously blue shift and randomly blink. The centroid of a QD is easily simulated when only one QD is “on.” After the centroids of both QDs are fitted, the distance between the two QDs is determined by the spacing of DNA. The distances between two QDs were measured using three different lengths of DNA rulers (15bp, 30bp, and 45bp). The measured distances agree well with the calculated ones, which demonstrate the feasibility of the approach.
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