Abstract
Botrytis cinerea affects plant yield and quality. Many Botrytis species are morphologically similar leading to difficulty in pathogen identification. Spectroscopy can be used to identify pathogenic fungi. This study describes a novel method for fungal characterization. Here, we determined the spectral signatures of different B. cinerea isolates as well as various fungal genera. A unique spectral pattern was investigated at both genus and isolate level. The short wave infrared II (2055 - 2315 nm) provided the best discrimination between the fungal samples observed. Moreover, the spectral analysis was performed on non-transformed data and investigated significant differences among fungal genera as well as B. cinerea isolates, while the results investigated high similarity among replicates of the same isolate of B. cinerea. The results of each spectral test were obtained reproducibly without an expensive cost consumable during sample preparation and measurements. This innovative approach would allow us to identify, discriminate and classify fungi rapidly and inexpensively at the genus, species and isolate level.
Highlights
Botrytis cinerea Pers.:Fr. (teleomorph: Botryotinia fuckeliana Whetzel), the cause of gray mold, is a necrotrophic pathogen causing economically important pre- and postharvest diseases in at least 235 plant species
We determined the spectral signatures of different B. cinerea isolates as well as various fungal genera
The spectral analysis was performed on non-transformed data and investigated significant differences among fungal genera as well as B. cinerea isolates, while the results investigated high similarity among replicates of the same isolate of B. cinerea
Summary
Botrytis cinerea Pers.:Fr. (teleomorph: Botryotinia fuckeliana (de Bary) Whetzel), the cause of gray mold, is a necrotrophic pathogen causing economically important pre- and postharvest diseases in at least 235 plant species. Variation in morphology and aggressiveness characteristics had been reported between isolates of Botrytis spp. [1,2], many species are morphologically similar [2]. Genetic characterization of B. cinerea had showed significant differentiations among isolates collected from different host plants [7,8]. It was previously determined that isolates sampled from grapes and tomatoes were genetically differentiated using random amplified polymorphic DNA (RAPD) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) markers [9]. Discrimination of fungi at species-level had been investigated using ITS rDNA test, but variation in the ITS region within Botrytis is low, limiting its use in this genus [3]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
