Spectral-Domain Phase Microscopy for Thickness Encoded Suspension Array

Abstract

A system containing spectral-domain phase microscopy and fluorescence microscopy has been developed to decode and detect thickness-encoded suspension arrays. The spectral-domain phase imaging path is used for decoding the thickness of the micro glass pieces while the fluorescence imaging path is used for quantifying the targeted analytes. The spectral-domain phase microscopy uses a spectral domain phase retrieval technique so that it has a high-dynamic thickness imaging range and submicron-scale thickness resolution, increasing the encoding efficiency and decoding precision. The thickness decoding capability of the system has been demonstrated by multiplexed immunoassay experiments. The quantitative analysis capability has been verified by concentration gradient experiments.