Abstract

The computer screen photoassisted technique (CSPT) is a practical method for the classification of colored or fluorescent substances such as those present in bioassays but using standard computer sets and regular web cameras as all instruments. It is empirically known that the performance of the CSPT classification depends on the composition of the illuminating sequences displayed by the computer screens during the measurements, and by the way that substance fingerprints from these measurements are composed. Here the illuminating conditions are examined with the help of a CSPT model that allows to examine the physical grounds of optimum illuminating sequences different from just three screen primaries and to assess the limitations and importance of longer illuminating sequences in practical determinations.

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