Abstract
The behaviour of bacteriochlorophyll in its natural state with regard to oxidative bleaching, photobleaching and temperature bleaching was investigated. Reversible changes in absorption by the addition of oxidative and reductive agents, previously measured in organic solution, were also found to be present when bacteriochlorophyll is in its natural state in chromatophores of purple bacteria. Only the absorption band of longest wavelength in Rhodospirillum rubrum, Rhodopseudomonas spheroides and Chromatium could be bleached reversibly. Also a reversible shift in position of the 800-mμ absorption band in Rh. rubrum occurs while there are indications of a similar shift in the other species. At a potential of approximately 515 mV the difference spectrum obtained by addition of ferri-ferrocyanide mixtures to chromatophores of Rh. rubrum was similar to the difference spectrum obtained by Duysens by illumination of living bacteria. Additional bleaching experiments were done by heating and illumination of the chromatophore extracts. The present experiments may yield a link between the in vitro and in vivo phenomena obtained with chlorophyllous pigments.
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