Spectral Analysis of Nanodiamond-Berberine Complex Interaction with Living Cells for Nanoparticle Mediated Drug Delivery

Yu-Chung Lin,Lin-Wei Tsai,Chia-Liang Cheng,Zhe-Rui Lin,Elena Perevedentseva,Artashes Karmenyan

doi:10.18287/jbpe17.03.010305

Yu-Chung Lin, Lin-Wei Tsai + Show 4 more

Open Access

https://doi.org/10.18287/jbpe17.03.010305

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Abstract

The natural isoquinoline alkaloid berberine has been demonstrated for its significant activity against a variety of deceases caused by bacterial and parasite infections. Nanodiamond (ND) has also been proven to have great potential for drug delivery. In this work, the complex of ND with berberine is constructed through surface functionalized ND, aiming for drug delivery applications. Spectroscopic analysis was performed on the complex interaction with human lung alveolar carcinoma epithelial cell (A549) and red blood cell (RBC) in comparison with berberine in solution. The studied cells were treated with carboxylated ND, ND-berberine complex and berberine, respectively. Their distributions in cells structures are visualized using ND and berberine’s fluorescence signals for detection. The distribution of ND and ND-berberine complex differs with the distribution of berberine introduced in solution form. The results show that the ND with attached drug molecules can act as a drug carrier allowing controllable localization of the drug into the cell. This result can have implication in drug delivery, release and delivery tracing applications.

Highlights

Nanodiamond (ND) is recently considered to be a promising material for various bio-medical applications due to its unique features in sizes, structure, surface chemistry, physical properties and biocompatibility [1, 2] the optical-spectroscopic properties of ND such as Raman and fluorescence [3, 4] open wide possible applications for bio-imaging and bio-sensing [5]
The difference in the solutions spectra intensities is proportional to quantity of the substance which was removed from the solution in the result of the interaction with nanodiamond [33]
One can see that significant quantity of the berberine is washed out from the nanodiamond, especially at 2nd washing

Summary

Introduction

Nanodiamond (ND) is recently considered to be a promising material for various bio-medical applications due to its unique features in sizes, structure, surface chemistry, physical properties and biocompatibility [1, 2] the optical-spectroscopic properties of ND such as Raman and fluorescence [3, 4] open wide possible applications for bio-imaging and bio-sensing [5]. Develop methods of delivery using ND implies analysis of functionality of bio-active molecules attached on ND [11, 12] and studies of mechanism of ND internalization by cells. Nanodiamond’s properties and surface modification with bio-active molecules can alter the ND penetration into cell [15]; this effect can be used to control the internalization of ND in cells. Difference in mechanism of interaction of nanodiamond conjugates and pure drug with the target objects can modify the treatment [18], to overcome the chemoresistance of tumor [19, 20]. Using ND based conjugates allows improving efficiency of the treatment and decreasing the possible side effects

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