Spectral analysis of basal cell cancer in vivo.

Abstract

One of the most important features of optical biopsy methods is its non-invasive nature, high sensitivity and resolution in comparison to traditional diagnostic methods. Optical diagnostic methods, contrary to histopathological tests and biochemical analysis, do not require the collection of tissue samples for analysis, and the amount of analyzed material is practically unlimited. The aim of this study was to evaluate the usefulness of spectral studies in the diagnostics of skin basal cell cancer using a protoptive device in clinical conditions. Each of the subjects was measured 4 times. The measurements were performed on the skin of a patient without a photosensitizer and on the healthy skin on the opposite side of the change in relation to the middle line, also without a photosensitizer. Patients were then given photosensitizer in form of ointment from ALA (Sigma Aldrich, USA) and lubricated with this ointment with occlusal dressing. The patient was examined again 3 h after the application. In each case the measurement time was 1.2 s. Significant differences were observed for the measurements of the sick patient's skin with photosensitizer. Significant differences in spectral curve between 570 and 780 nm were observed.