Speckle-free laser projection structured illumination microscopy based on a digital micromirror device

Abstract

In the structured illumination microscopy (SIM) family, interferometric implementations using traditional physical gratings or advanced liquid crystal on silicon spatial light modulators are popular but have the disadvantages of expensive and limited speed. A promising alternative of a digital micromirror device (DMD) has improved the situation for its lower cost and higher speed, furthermore, it has contributed to the proposal of a simple and compact projection DMD-SIM. However, the projection DMD-SIM method is accused of erroneously measuring the resolution because of the scattering characteristic of gold beads, despite the inferior experimental resolution that is because of the low fringe contrast and the resulting low signal-to-noise ratio (SNR). As an improvement, we use a high-brightness laser for illumination to achieve higher SNR, and the accompanying problems of speckle and inhomogeneity originating from the laser’s high coherence are solved by a high-frequency phase-randomization deformable mirror in conjunction with a square-core multimode fiber, which does not limit the image acquisition speed and with fewer energy losses compared to the existing methods. Also, in terms of the problem of low SNR, we apply a parallel-iteration Richardson–Lucy deconvolution algorithm for SIM reconstruction to further improve the imaging performance based on ensuring the resolution. Based on these advances, we image fluorescent nanoparticles and other non-biological and biological fluorescent samples, and the experimental results demonstrate the improved imaging performance of our modified system.