Abstract
Moutan Cortex (MC) has been demonstrated to have an inhibitive effect on inflammation and oxidative stress responses in mesangial cells in our previous study. However, little is known about the components of MC contributing to this benefit. In the present study, cell membrane immobilized chromatography (CMC), a fast and useful method, was presented for screening potential active components of MC. HBZY-1 cells were incubated with MC (200 μg/mL) at the optimal incubation time (90 min). HPLC-DAD analysis and LC/ESI/MS/MS were performed to distinguish the active components and identify its structural ion fragments. The results showed that eight components binding to HBZY-1 cells were mudanoside B, paeoniflorin sulfonate, paeoniflorin, tetragalloyl glucose (isomeride), hexagalloyl glucose, mudanopiside A, and paeonol. In conclusion, our established CMC might be a useful method for screening potential active components in complicated traditional Chinese medicines. These components might be associated with the efficacy of MC on prevention and treatment of diabetic nephropathy.
Highlights
Diabetic nephropathy (DN), one of microvascular complications in diabetic patients as the end-stage renal disease, is the leading cause of high morbidity and mortality of diabetes mellitus (DM) throughout the word [1]
As shown in Figure 2(A) and Figure 2(B), after being incubated with three concentrations of Moutan Cortex (MC), the most components of MC had a high peak area at the concentration of 200 μg/mL. It indicated that MC of 200 μg/mL had a better interaction with HBZY-1 cells
Studies show that cell membrane immobilized chromatography (CMC) is a powerful tool for screening of active compounds in traditional Chinese medicines (TCMs) extract relying on the living cells incubated in the culture flask associated with the pathogenesis through the relevant passageway
Summary
Diabetic nephropathy (DN), one of microvascular complications in diabetic patients as the end-stage renal disease, is the leading cause of high morbidity and mortality of diabetes mellitus (DM) throughout the word [1]. It has been investigated that paeoniflorin and oxypaeoniflorin have the antioxidant and anti-inflammation activities on AGEs-induced mesangial cell damage in our present study [13]. The use of CMC by biomembrane of organism extraction together with chromatography analysis may be a powerful tool for screening potential active components in TCMs. the present study was conducted to establish an appropriate CMC method for screening the potential active components in MC which interacted with mesangial cells. The present study was conducted to establish an appropriate CMC method for screening the potential active components in MC which interacted with mesangial cells These active components, which were related to the protective effect of MC on DN, were revealed for the first time in our study
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