Abstract

IN an earlier publication1 we reported that vitamin B12 could not be replaced for Euglena gracilis var. bacillaris by vitamin-free casein hydrolysate, carbon-treated peptone, methionine, ascorbic acid, pteroylglutamic acid, a variety of purine and pyrimidine bases or by xanthopterin and a number of related compounds. Hutner et al.2 found thymidine to be ineffective. Vitamin B12a (hydroxocobalamin) was active1. We have examined a number of additional compounds for vitamin B12 activity on Euglena.

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