Specificity of association of a [formula omitted] ATPase with cholinergic synaptic vesicles from [formula omitted] electric organ

Abstract

Cholinergic synaptic vesicles from the electric organ of Torpedo californica have been subjected to analytical scale separation techniques not utilized in the isolation procedure, and the ATPase activity of separated fractions determined. Most of the ATPase activity migrated with the vesicles. Sensitivity of the ATPase activity to 16 potential inhibitors also was determined. Most of the ATPase activity was inhibited by low concentrations of 4-chloro-7-nitrobenzo-oxadiazole (NBD-C1) and dicyclohexylcarbodiimide (DCCD), but not by a water soluble carbodiimide. The close association of the ATPase with the vesicles and the pattern of inhibition obtained provide further support for the authentic presence of a membrane bound Ca 2+ Mg 2+ ATPase in the cholinergic synaptic vesicle.