Specificity of Antibodies Produced by Linear Antigenic Polypeptides of a Known Primary Structure: Synthesis and Use of the Polymers Poly(L-phenylalanyl-L-glutamyl-L-valylglycyl)glycine Methyl Ester and Poly(L-phenylalanyl-L-aspartyl-L-valylglycyl)glycine Methyl Ester

Abstract

The syntheses of the polypeptides poly(L-phenylalanyl- L-glutamyl-L-valylglycyl)glycine methyl ester and poly(L-phenyl- alanyl-L-8spartyl-L.-valylglycyl)glycine methyl ester are described. Using these materials, it was found by cross-reaction and absorption studies that the antibodies to the antigen poly(L-tyrosyl- L-glutamyl-L-alanylglycyl)glycine-l-14C ethyl ester show specificities for the phenolic hydroxyl group of the tyrosyl residue and the y-carboxyl group of the glutamyl residue.