Abstract
Fast and accurate detection of specific IgE (sIgE) is essential for diagnosis of allergic diseases. In this study, we developed a nano-capturer Fe3O4@SiO2-NTA based on magnetic nanoparticles with surface modification of Ni-NTA moieties. Results showed that our immunosensor based on the specifically immobilization of recombinant allergen protein on the Fe3O4@SiO2-NTA could realize fast, high efficiency and low-cost detection of sIgEs in serum samples. In vitro experiments demonstrated that the chemiluminescence (CL) response of the immunosensor to sIgEs showed an obviously discrimination between positive and negative serum samples. The CL intensity values were proportional to the sIgE concentrations in the range of 2.52–14.02 ng/mL with detection limit of 0.35 ng/mL, which offered a promising platform for fast and quantitative determination of sIgEs. Furthermore, our method was successfully utilized for allergic disease diagnosis in 46 serum samples and satisfied results were achieved in comparison with the commercial ELISA kit. In particular, by simply changing various recombinant allergen proteins immobilized on the surface of Fe3O4@SiO2-NTA, our strategy showed great potential for high-throughput analysis of allergen screening.
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