Specific resistance to soybean stem canker conferred by the Rdm4 locus

Abstract

The Rdm4 gene from soybean cv. Hutcheson has been extensively used to incorporate resistance to soybean stem canker (SSC), caused by Diaporthe phaseolorum var. meridionalis (Dpm), into soybean commercial cultivars. The objective of this work was to characterize the inheritance of the Rdm4 locus in different populations derived from the cross: J77‐339 (rdm/rdm, susceptible) × Hutcheson (Rdm4/Rdm4, resistant) in independent interactions with two local isolates of Dpm. Four F2 populations were obtained and two were advanced to the F3 generation as separate F2:3 families to perform progeny tests. Each population was inoculated with the CE109 and/or CE112 isolates of Dpm. Within each plant–pathogen interaction, the resistance gene segregated as completely dominant. However, cross resistance, or opposite disease reactions, to CE109 and CE112 isolates of Dpm were observed in four F2:3 families, indicating an intergenic recombination event between two nonallelic genes interacting specifically with each isolate of Dpm. The distance between them, estimated as the recombination fraction, was 29%, suggesting that both genes were not tightly linked, but close enough to segregate together in most crosses. Results indicated the existence of a genomic region in cv. Hutcheson composed of race‐specific resistance loci with at least two Rdm genes: the previously recognized Rdm4 and a novel gene, tentatively named Rdm5, conferring specific resistance to Dpm isolates CE109 and CE112.