Specific polysiloxane-based fluorescent probe for imaging ONOO− in lipid droplet

Abstract

Peroxynitrite (ONOO−) is a highly reactive nitrogen species formed by the combination of superoxide anion radicals and nitric oxide radicals. However, lipid droplets, which are important cellular organelles involved in metabolism, contain a variety of enzymes. The presence of high concentrations of ONOO− can lead to the inactivation of enzymes and other substances within lipid droplets, resulting in various diseases. Therefore, it is imperative to develop methods for detecting ONOO− in order to understand its physiological and pathological mechanisms. In this study, we have successfully developed a polysiloxane platform by modifying the side-chain NH2 group. This platform incorporates naphthylimide, which exhibits an ICT (intramolecular charge transfer) mechanism, as well as triphenylamine derivatives that specifically target lipid droplets. By utilizing this polysiloxane platform, we have created a fluorescent probe (S-YHS) that selectively and sensitively detects ONOO− within lipid droplets. The development of this lipid droplet-targeting ONOO− fluorescent probe has significant implications for understanding the role of ONOO− in various diseases. Its high selectivity and sensitivity make it a valuable tool for studying the physiopathological mechanisms associated with ONOO−.