Specific Parameters of Detection of Fungi of the Genus <i>Aspergillus</i> in Respiratory Organs of Pulmonary Tuberculosis Patients

Abstract

The objective: to study specific parameters of detection of fungi of the genus Aspergillus in respiratory organs of pulmonary tuberculosis patients.Subjects and Methods. In 2019-2021, the samples of sputum or bronchoalveolar lavage of 520 patients above 18 years old with confirmed pulmonary tuberculosis and HIV-negative status were tested for molds. Also, all patients had their sputum or BAL tested for acid fast mycobacteria (AFB), cultures of M. tuberculosis (MBT), and DNA of tuberculous mycobacteria.Results. Positive culture results for fungi were obtained in 50/520 (9.62%; 95% CI 7.37-12.45, Wilson test) pulmonary tuberculosis patients. Species identification revealed fungi of the genus Aspergillus in 46/50 (92%) of them.In 37/46 (80.43%; 95% CI 66.83-89.35) patients, a single type (one species of Aspergillus) was identified, in 9/46 (19.57%; 95% CI 10.65-33.17) two types were identified. A. fumigatus (16/37 isolates, 43.24%) and A. niger (13/37 isolates, 35.14%) prevailed among single types of cultures. A. niger + A. flavus (3/9 isolates, 33.33%) was the most frequent combination. The ratio of the effectiveness of methods for detecting fungi such as culture/direct microscopy/galactomannan antigen in serum and BAL was 100/84.78/4.35% and 67.39%, respectively. Thus, BAL is more preferable than serum for detection of galactomannan antigen in patients with tuberculosis.Only in 8/46 (17.4%) patients, bacterial excretion was detected by culture, which is typical for the active phase of the course of tuberculosis. In the remaining 38 (82.6% 95% CI 69.28-90.91) patients, no tuberculous mycobacteria were detected by culture, which was typical of stabilization and cure of tuberculosis.